CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

Conventional in vitro propagation may affect normal morphophysiological characteristics and survival during the acclimatization of commercial plants, such as Etlingera Elatior (Jack) R.M. Smith. CO2 enrichment, gas-permeable membranes, reduced carbohydrate concentration culture media can be used to mitigate these effects. Here, we aimed determine impact photomixotrophic photoautotrophic growth on responses E. elatior its ex acclimatization. In vitro-established plants were placed flasks containing semisolid MS medium with 0.54 µM α-naphthalene acetic acid sucrose (0 or 15 g L−1). The sealed lids without orifices covered by membranes kept for 45 days under forced-air ventilation (360 1000 µmol−1 CO2). Growth variables, anatomical physiological characteristics, rate evaluated. Overall, µmol mol−1 CO2, L−1 resulted higher fresh dry mass, level photosynthetic pigments, well more developed secondary ribs leaves, prominent midribs, highly organized differentiated chlorenchyma cells (palisade spongy parenchyma), vascular bundles, intercellular gaps reduced, compared conventionally vitro-propagated plants. Moreover, conditions promote favored (75%) when transferred conditions.